Why put ANALIZA on your team for
LogD?
Analiza measures LogD directly, using its thermodynamic definition: the partition coefficient of buffer-octanol system in equilibrium using a novel, automated and miniaturized version of the gold standard shake-flask method.
Manual partitioning experiments require very long mixing and phase separation – typically overnight. Due to the small volume of our partitioning experiments, the unique way in which we vortex, and the use of centrifugation to achieve phase separation, our phase equilibrium is achieved very fast, allowing us to deliver results fast.
Large dynamic range -3< LogD < +4
- Without diluting or concentrating
Archival quality data
- True LogD, not an approximation
Dry and DMSO-dissolved compounds
- LogD is corrected for DMSO
Multiple pH: 2, 4, 6, 7.4, 11
- Early screening: Test many compounds at one pH
- Lead optimization: Test a few compounds at multiple pH
Compound structure is not needed
Our universal method of detection means we only need to know the number of Nitrogen atoms per molecule and its formula weight
Only a small amount of compound is needed
We don’t waste your valuable compounds creating compound-specific calibration curves
Fast turnaround time
- Our universal process means that we can provide archival data within 48 hours of receiving your compounds
Highest level of accuracy and repeatability
- Routine complete-system calibration
- Real-time monitoring of all the system’s critical performance parameters
- Assays performed with an on-board set of standards
Extensive validation
- We have done extensive validation studies with hundreds of compounds and have shown that our automated method produces results that are very tightly correlated to manual shake-flask data. Using the same buffer composition at the same pH, a linear relationship with a one percent deviation in the slope from the theoretical slope of one was determined, over the range of LogD from –3 to +4. Both short-term and long-term precision studies were conducted, and the 3 standard deviation (95% confidence interval) was about 0.2 LogD units, when measured from 45 repeated measurements.
LogD Quick Facts
Assay: 1-octanol water partition coefficient in universal buffer
Method: Automated, miniaturized gold standard shake-flask method with equimolar chemiluminescent nitrogen detection of both octanol and aqueous phases
Available pH: 2, 3.5, 4.4, 6.6, 7.4, 11
Experience: Developer of Automated ADME technologies since 1997 with tens of thousands of data points since 2005 for clients ranging from large pharma to small biotechs
LOD: -3<LogD<+4 (Nominal, actual compound-specific limits are determined by compound amount, molecular weight, and number of nitrogen atoms in each compound)
Accuracy: Linear fit between automated manual shake-flask and literature data demonstrated fit with r2 =0.996
Precision: Typically SD < 0.15 log units
Failure Rate: 0.3-0.7% based on over 12,000 data points
Source: Dry Powder or DMSO-dissolved compounds in 96 well plates, typically 50µL, 30mM concentration
Turnaround time: As little as 24 hours, typically within 5 business days
Results: Provided electronically. Tabulated data in Excel per customer instructions. All results are analyst-checked; internal standards are included on each plate.
