pKa
Assay information
Method
24-point Capillary Electrophoresis
Compound Requirements
30μL 10mM DMSO stock
Compound Concentration
100μM
Analytical Range
pKa 1.8 – 11.2
Data Delivery
pKa(s)
24-point titration curves
pKa plays a significant role in a compound’s solubility, lipophilicity, and permeability
Capillary electrophoresis allows us to obtain pKa measurements for compounds with low solubility using a cosolvent approach. Unlike the potentiometric method, measurements can be obtained for compounds with chromophores located further away from the center of ionization.
Capillary electrophoresis separations are carried out in parallel across 24 equally spaced pH’s between 1.8 and 11.2. The compound migration time is monitored relative to a neutral marker (DMSO), and a titration curve is generated from this data.
An internal validation study was recently conducted to evaluate pKa measurements obtained at Analiza with those the from literature*.
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