Physicochemical Properties

Early-stage drug discovery hinges on accurate, high-quality data about each compound’s fundamental characteristics. At Analiza, we’ve miniaturized and automated gold-standard assays to measure solubility, logD/logP, pKa, and more—providing quick, reliable insights that guide your pipeline decisions.

Why Physicochemical Data Matters

Physicochemical properties shape how a molecule behaves in both in-vitro and in-vivo environments. By understanding factors like solubility, lipophilicity, and pKa, you can optimize a compound’s efficacy, formulation, and safety profile far earlier in the development process.

Physicochemical Property Assays

AlphalogD

Designed for beyond-rule-of-5 (BRo5) compounds, AlphalogD is a chromatography-based assay that extends beyond traditional logD measurement. It reliably captures highly lipophilic or large molecule profiles, guiding medicinal chemists working in emerging chemical spaces.

EPSA

EPSA is defined as a shielded polarity index measured by supercritical fluid chromatography (SFC) using a polar, Pirkle column system. Developed for macrocycles and other potential large, flexible molecules to highlight intramolecular hydrogen bond (IMHB) and chameleonicity.

pKa

Using capillary electrophoresis, we can determine a compound’s ionization constants even for low-solubility molecules. Knowing your compound’s pKa is crucial for predicting solubility, permeability, and overall ADME performance in various pH environments.

Solubility

We use a miniaturized shake-flask approach for thermodynamic solubility, quantifying results with CLND, HPLC-UV, or HPLC-MS. With as little as 15 μL of 10 mM stock, this assay yields precise measurements that help predict in-vitro success and ensure clean downstream data.

Want to learn more about our services?

Contact our team today to see how Analiza can accelerate your drug discovery program.