Miniaturized Shake-Flask LogD/LogP

Whether you’re tight on budget, time, or internal capacity Analiza offers a logD/logP assay to meet your needs. We understand the fast timelines and scarce compound availability associated with discovery operations so we scaled down the gold standard shake-flask method to deliver accurate, uncompromised data from just 30 μL of 10mM DMSO stock solutions on your schedule.

Single point assays provide robust rank order classification of compounds under physiologically relevant conditions. For a more thorough investigation into their in vitro behavior our vast library of proprietary partitioning systems allows us to quickly and affordably deliver true shake-flask pH-logD profiles across the physiologically relevant pH range without buffer composition artifacts.

Using measured pKa data, logP is determined under conditions at which the compound is neutral.

The data below illustrates the ruggedness and reproducibility of our miniaturized, gold standard shake-flask method. In a snapshot of over 100 logD studies executed in octanol-PBS, pH 7.4 at Analiza, the on-board performance indicating standards of harmine and sulfamethizole illustrate the long term inter-assay reproducibility of our shake-flask logD assay; Harmine, for example yielded an average measured logD of 2.89 at pH 7.4.